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KMID : 0364820100460010086
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Korean Journal of Microbiology
2010 Volume.46 No. 1 p.86 ~ p.92
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Isolation and Characterization of Keratinolytic Protein Chicken Feather-Degrading Bacteria
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Kim Se-Jong
Cho Chun-Hwi
Whang Kyung-Sook
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Abstract
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Thirty-one chicken feather-degrading bacteria were isolated from wasted feather, compost and wastewater in a chicken farm. These isolates were categorized as Firmicutes (21 strains), γ-proteobacteria (4 strains), Actinobacteria (4 strains), and Bacteroidetes (2 strains) by 16S rRNA gene sequence analysis. We examined the feather-degrading isolates for degradation in the 2% of chicken feather meal. The strain Chryseobacterium sp. FBF-7, Stenotrophomonas maltophilia FBS-4, and Lysinibacillus sp. FBW-3 were selected as a keratinolytic protein degrading bacteria which showed the highest feather degradation of 75-90%. The characteristics of amino acids extracted from chicken feather meal by using keratinolytic protein degrading isolates and chemical method with Ca(OH)2 were analyzed. Total amino acid content of strain Chryseobacterium sp. FBF-7 was 1,661.6 μmol/ml, which was the highest and it was similar with chemical method. And essential amino acid content of total amino acid was thirty-seven percent (619.3 μmol/ml) and 596.9 μmol/ml for keratinolytic protein degrading isolates and chemical method, respectively. The major amino acids were valine, glutamic acid, aspartic acid, glycine, and proline by the strain Chryseobacterium sp. FBF-7 and especially, higher contents of
aspartic acid, threonine, serine, cysteine, and tyrosine were detected compared with chemical method.
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KEYWORD
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amino acids, chicken feather, keratinolytic protein degrading bacteria
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